Now just to make sure you’ve understood everything, here’s a quiz to test your memory!! All the answers are in the text so if you get stuck just look back.

1) What is the structure of the endoplasmic reticulum and where is it found in the cell?

2) What is the function of the Smooth ER?

3) What is the function of the Rough ER?

4) What is the purpose of the signal recognition particle?

5)Describe the sequence of steps that occur when a ribosome encodes for a protein that contains a signal sequence

6) What is this process called?

7) What is Post-Transitional transport? Give an example of a eukaryote that does it.

8) Name the four main modifications carried out by the ER

9) In which part of the ER does protein folding occur?

10) What is Transitional ER?

11) What are the 2 main purposes of the protein coat that surrounds the vesicles formed from the ER?

12) What are the roles of the chaperone proteins Calreticulin (CRT) and Calnex (CNX)?

13) Describe the steps in vesicle budding, starting from the activation of the G protein Sar1, found in the COPII protein

14) What percentage of proteins are improperly folded in the ER?

15) What is the process of Dislocation?

16) What is the function of N-glycanase?

17) What is the difference in functions of the enzymes Glycosidase I & II and Glucosidase II?

18) What causes a Heat Shock Response and how is it dealt with by the cell?

19) What is the Unfolded Protein Response and how is it dealt with by the cell?

20) How many steps are involved to remove misfolded proteins during the Unfolded Protein Response in Yeast cells?

21) What action does the UDP-glucose glycoprotien glycosyl transferase do?

22) What is the function of the Transmembrane Kinase?

23) Why is the mRNA translated during these steps?

24) Where do the vesicles from the ER go to finally after processing?

25) How is the addition of sugars (eg glucose, mannose) used by the ER to measure time in relation to folding proteins?